Chemometric-assisted UV-spectrophotometric method for simultaneous estimation of paracetamol, chlorzoxazone and ibuprofen in multicomponent formulation
AshEse Journal of Pharmacy
Vol. 1(1), pp. 001-008, June, 2016
© 2016 AshEse Visionary Limited
Full Length Research
Santosh V. Gandhi* and Shreeyash R. Tapale
Department of Quality Assurance, AISSMS College of Pharmacy, Kennedy Road, Pune- 411001, Maharashtra, India.
*Corresponding author. E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.. Tel: +91-9689932181, +91-20-26058208.
Received April, 2016; Accepted June, 2016
The presented work is based on chemometric-assisted UV-spectrophotometric method of quantitative analysis for simultaneous estimation of Paracetamol, Chlorzoxazone and Ibuprofen in combined pharmaceutical dosage form. The chemometric methods used were Principal Component Regression (PCR) and Partial Least Square (PLS). The Spectra of the drugs were recorded at concentrations within their linear concentration range of 3.0-18.0 μg/ml, 2.5-12.5 μg/ml and 4.0-24.0 μg/ml for Paracetamol, Chlorzoxazone and Ibuprofen in methanol, respectively. Calibration set was designed using multilevel multifactorial design. 28 set of mixtures were used for calibration set and 12 set of mixtures were used for validation set in the wavelength range of 215 to 295 nm with the wavelengths intervals λ= 0.5 nm. The methods were validated as per International Conference on Harmonization Q2 (R1) (ICH) guidelines. These methods were successfully applied for determination of drugs in pharmaceutical formulation (tablet) with no interference of the excipients as indicated by the recovery study results. The proposed methods are simple, rapid and can be easily used as an alternative analysis tool in the quality control as well as in process control of drugs and formulation.
Key Words: Paracetamol, Chlorzoxazone, Ibuprofen, PLS, PCR, Validation.